Nesi Nathalie

Dr. Nathalie NESI– Research scientist at INRAE Rennes, France – Joint Laboratory for Genetics, Environment and Plant Protection (IGEPP)

https://cv.archives-ouvertes.fr/nathalie-nesi

Contact address

UMR1349 IGEPP, INRAE – Agrocampus Ouest – Université Rennes1, Domaine de la Motte, BP 35327, 35653 Le Rheu Cedex, France

Date of birth: 9^th August 1974

Career

Since 2003:    INRAE scientist (CR1) at INRAE Rennes (France)

2001 – 2002:   Postdoctoral fellow at JIC Norwich (UK), Department of Metabolic Biology

1997 – 2001:   PhD in Plant Sciences at INRA Versailles (France), Laboratory of Seed Biology

1996 – 1997:   Advanced master degree in Plant Cellular and Molecular Physiology

1994 – 1996:   Diploma from the National School for Agronomy Paris-Grignon

Research interests and Expertise

My research project is part of the team entitled “ Yield under Abiotic Challenges ” and focuses on seed development and maturation. I have a particular interest in unravelling the genetic and molecular determinants involved in the control of oil content as well as in the development of integuments, especially the accumulation of flavonoids in the seed coat.

I am working on the following topics: 1) genetic and molecular analyses of seed oil content, 2) improvement of meal digestibility by modifying the seed phenylpropanoid content, 3) analysis of the impact of oil body protein composition on oil extraction potential and 4) monitoring of gene expression during seed development in Brassica napus. I have also developed an EMS-mutagenised population of OSR for reverse genetic approach RAPTILL . I have been involved in several national (Génoplante) or transnational (GABI-Génoplante) projects.

I coordinated the ANR-Genoplante project GENERGY and I am currently coordinating the RAPSODYN project (Investments for the Future, 2012-2019).

Publications

Jolivet P, Boulard C, Bellamy A, Valot B, d'Andréa S, Zivy M,Nesi N, Chardot T. Oil body proteins sequentially accumulate throughout seed development in Brassica napus. J Plant Physiol (2011) PMID: 21803444.

Auger B, Marnet N, Gautier V, Maia-Grondard A, Leprince F, Renard M, Guyot S,Nesi N, Routaboul JM. A detailed survey of seed coat flavonoids in developing seeds of Brassica napus L. J Agric Food Chem (2010) 58: 6246-6256.

Auger B, Baron C, Lucas MO, Vautrin S, Bergès H, Chalhoub B, Fautrel A, Renard M,Nesi N. Brassica orthologs fromBANYULSbelong to a small multigene family, which is involved in procyanidin accumulation in the seed. Planta (2009) 230: 1167-1183.

Jolivet P, Boulard C, Bellamy A, Larré C, Barre M, Rogniaux H, d'Andréa S, Chardot T,Nesi N. Protein composition of oil bodies from mature Brassica napus seeds. Proteomics (2009) 9: 3268-3284.

Nesi N, Lucas MO, Auger B, Baron C, Lécureuil A, Guerche P, Kronenberger J, Lepiniec L, Debeaujon I, Renard M. The promoter of the Arabidopsis thaliana BANgene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat. Plant Cell Rep (2009) 28: 601-617.

Pignocchi C, Minns GE,Nesi N, Koumproglou R, Kitsios G, Benning C, Lloyd CW, Doonan JH, Hills MJ. ENDOSPERM DEFECTIVE1 is a novel microtubule-associated protein essential for seed development in Arabidopsis. Plant Cell (2009) 21: 90-105.

Nesi N, Delourme R, Brégeon M, Falentin C, Renard M. Genetic and molecular approaches to improve nutritional value of Brassica napus L. seed. C R Biol (2008) 331: 763-771. PMID: 18926490.

Devouge V, Rogniaux H,Nesi N, Tessier D, Guéguen J, Larré C. Differential proteomic analysis of four near-isogenic Brassica napus varieties bred for their erucic acid and glucosinolate contents. J Proteome Res (2007) 6: 1342-1353.

Lepiniec L, Debeaujon I, Routaboul JM, Baudry A, Pourcel L,Nesi N, Caboche M. Genetics and biochemistry of seed flavonoids. Annu Rev Plant Biol (2006) 57: 405-430.

Debeaujon I,Nesi N, Perez P, Devic M, Grandjean O, Caboche M, Lepiniec L. Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development. Plant Cell (2003)15: 2514-2531.

Nesi N, Debeaujon I, Jond C, Stewart AJ, Jenkins GI, Caboche M, Lepiniec L. The TRANSPARENT TESTA16locus encodes the ARABIDOPSIS BSISTER MADS domain protein and is required for proper development and pigmentation of the seed coat. Plant Cell (2002)14: 2463-2479.

Nesi N, Jond C, Debeaujon I, Caboche M, Lepiniec L. The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed. Plant Cell (2001) 13: 2099-2114.

Nesi N, Debeaujon I, Jond C, Pelletier G, Caboche M, Lepiniec L. TheTT8gene encodes a basic helix-loop-helix domain protein required for expression ofDFRandBANgenes inArabidopsissiliques. Plant Cell (2000) 12: 1863-1878.